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. 2024 Sep 11;27(10):110917. doi: 10.1016/j.isci.2024.110917

Figure 4.

Figure 4

Inhibition of NODAL signaling counteracts the effects of olanzapine on the promotion of stemness

(A) Sample images among multiple iPSC cell lines and clones for immunostaining of organoids at day 12. The immunofluorescence signal showing the spatial expression of PAX6, SOX2, and Nestin in 12-day CO treated by olanzapine-treated (Olan), olanzapine+SB431542 dual-treated (Olan+sb), control and SB431542 single-treated (Control+sb) groups. Scale bar, 20μm. (11–30 total rosette structures from at least three organoids).

(B) Sample images among multiple iPSC cell lines and clones for immunostaining of organoids at day 24.The immunofluorescence signal showing the spatial expression of PAX6, SOX2, and Nestin in 24-day CO treated by olanzapine-treated (Olan), olanzapine+SB431542 dual-treated (Olan+sb), control and SB431542 single-treated (Control+sb) groups. Scale bar, 20μm. (11–30 total rosette structures from at least three organoids).

(C–E) The results of the intensity of PAX6 (C), SOX2(D), and NESTIN (E) between four groups at day 12.

(F–H) The results of the intensity of PAX6 (F), SOX2(G), and NESTIN (H) between four groups at day 24.∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05, ns: p > 0.05. ANCOVA adjusting for three cell lines. Post-hoc analyses were conducted using the LSD adjustment method. Data are represented as mean ± SD. (Detailed statistical data can be found in Table S11).