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. 2024 Oct 28;15:9301. doi: 10.1038/s41467-024-53590-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — A Graph showing number of aggregating animals in npr-1(ad609) compared to npr-1;eat-4(ky5) mutants and number of aggregating animals in npr-1(ad609);eat-4(ky5) mutants with EAT-4 driven by srv-3, sra-6, nhr-79, and srv-3/sra-6 combined promoters. B Graph showing number of aggregating animals in npr-1(ad609), npr-1(ad609);nrx-1(wy778), npr-1(ad609);eat-4(ky5), npr-1(ad609);nrx-1(wy778);eat-4(ky5) mutants. Graph also includes npr-1(ad609);nrx-1(wy778);eat-4(ky5) mutants with EAT-4 driven under the nhr-79 promoter, npr-1(ad609);nrx-1(wy778);eat-4(ky5) mutants with NRX-1(α) driven under the nhr-79 promoter, and solitary controls. C Representative images of aggregation behavior in npr-1(ad609);eat-4(ky5), npr-1(ad609);eat-4(ky5); nhr-79p::eat-4, npr-1(ad609);nrx-1(wy778);eat-4(ky5), and npr-1(ad609);nrx-1(wy778);eat-4(ky5); nhr-79p::eat-4 animals (Scale bar = 1 mm). D Graph showing number of aggregating worms in npr-1(ad609), npr-1(ad609);eat-4(ky5), npr-1(ad609);nlg-1(ok259), npr-1(ad609);nlg-1(ok259);eat-4(ky5), npr-1(ad609);glr-2(ok2342), npr-1(ad609);nlg-1(ok259);glr-2(ok2342) mutants, and solitary controls. E Graph showing number of aggregating animals in npr-1(ad609), npr-1(ad609);nrx-1(wy778), npr-1(ad609);glr-1(n2461), npr-1(ad609);glr-2(ok2342), npr-1(ad609);avr-15(ad1051), npr-1(ad609);nrx-1(wy778);glr-1(n2461), npr-1(ad609);nrx-1(wy778); glr-2(ok2342), and npr-1(ad609);nrx-1(wy778);avr-15(ad1051) mutants. Data for npr-1 and npr-1;eat-4 are plotted in 4 A, 4B, and 4D. Data for npr-1;nrx-1 are plotted in 4B and 4E. Data for solitary controls are plotted in 4B, 4D, and 4E. The number of biological replicates (n) are displayed in the figure, bars show the mean number of aggregating C. elegans, and error bars indicate SEM. One-way ANOVA with Tukey’s post-hoc test was used for comparisons, exact p-values are shown on graphs (red indicates significance, black indicates non-significance). Source data are provided in the Source Data file.