FIG. 2.

DNA fingerprinting analysis. Southern blot hybridization patterns of 14 nosocomial C. parapsilosis isolates probed with the Cp3-13 probe (A) and 9 unrelated isolates (B). Samples of C. parapsilosis DNA were digested with EcoRI and SalI and electrophoresed in a 0.75% agarose gel. The molecular sizes of selected bands are indicated to the left of the gels. The reference strain, strain J940043, was analyzed in the outer two lanes to normalize the gel during computer-assisted analysis. (C) Dendrogram generated from similarity coefficients (S_AB) computed for pairwise comparisons of 26 nosocomial isolates and a collection of 16 unrelated isolates, including reference strain J940043, fingerprinted with probe Cp3-13.