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. 2024 Oct 28;10:454. doi: 10.1038/s41420-024-02220-y

Fig. 3. Xanthohumol promotes endogenous apoptosis in NSCLC drug-resistant cell lines.

Fig. 3

A HCC827OR and H1975OR cells were pretreated with z-VDA-fmk, necrostatin, or 3-MA for 4 h, then treated with xanthohumol (3 μM) for 24 h, and finally, the cell viability was analyzed by MTS assay. ***p < 0.001. The protein expression level of cleaved-Caspase 3 was detected by IB assay after 24 h of xanthohumol treatment; (B) caspase 3 activity was detected by caspase 3 activity assay kit for HCC827OR cells (C) and H1975OR cells (D); and c-caspase 3 positive cell number (E). Scale bar, 25 μM. ***p < 0.001. F Subcellular fractions were isolated for IB analysis After treatment of HCC827OR cells with xanthohumol for 24 h. G After treating HCC827OR cells with xanthohumol for 24 h, the number of apoptotic cells was analyzed by flow cytometry and statistical analysis was performed. *p < 0.05. **p < 0.01. ***p < 0.001.