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. 2024 Oct 28;10:454. doi: 10.1038/s41420-024-02220-y

Fig. 4. Xanthohumol suppresses osimertinib-resistant NSCLC cells by inhibiting the Ets-1/c-Met axis.

Fig. 4

After treating HCC827OR and H1975 cells with xanthohumol for 24 h, cells were collected for IB analysis (A, C) and qPCR analysis (B). *p < 0.05.**p < 0.01.***p < 0.001. D Knockdown of ETS and WB analysis of protein expression levels of Ets-1. E HCC827OR (top) and H1975OR cells (bottom) were treated with different concentrations of TK216 (ETS inhibitor, 0, 2, 4 μM), and the protein expression level of c-Met was analyzed by WB. HCC827OR and H1975OR cells were treated with xanthohumol, overexpressed Ets-1 or co-treated, and the protein expression levels of Ets-1 and c-Met were analyzed by WB (F); cell viability was analyzed by the MTS assay (G); colony-forming ability was detected by the soft-agar clone formation assay (H); and the caspase 3 activity assay kit to detect caspase 3 activity (I). Scale bar, 200 μm. ***p < 0.001.