FIG. 3.

Immunoblot analysis of serum samples from C. abortus-infected ewes. Serum samples from experimentally infected ewes A1 (a), A3 (b), and A6 (c) and the seroresponsive control ewe B1 (d) obtained throughout pregnancy were immunoblotted against whole EBs run on a 12% SDS-polyacrylamide gel. Ewes were challenged at 10 wg (a to c) and either aborted at 18 wg (a, b, and d) or lambed at 20 wg (c). For experimental details, see Materials and Methods. Molecular masses are indicated on the left. P and M indicate the 90-kDa POMP and 40-kDa MOMP antigens, respectively (25, 27). This figure was prepared from digital photographic images using CorelDRAW version 9.