FIG. 4.

Suppression of the lysis phenotype of hsf1-ba1 cells by osmotic stabilization. (A) Growth of cells containing hsf1-ba1 and wsc1Δ mutations under various conditions. Wild-type HSF1 cells (WT) and their derivatives containing hsf1-ba1 and wsc1Δ mutations were streaked onto YPD medium or YPD medium containing 1 M sorbitol and were incubated at 28, 35, or 38°C for 2 days. (B) Cell lysis assay of hsf1-ba1 and wsc1Δ cells grown at elevated temperature. Suspensions of the indicated cells were spotted on YPD medium or YPD medium containing 1 M sorbitol. Plates were incubated at 28°C for 1 day and then switched to 38°C and incubated overnight. The plate was overlaid with an alkaline phosphatase assay solution and incubated at 38°C for 1 h.