FIG. 2.
Phylogenetic tree of the eukaryotic GLF family ORFs. A minimum evolution tree for the predicted protein sequences of candidate eukaryotic GLFs was constructed using algorithms implicated in the MEGA3 software package (11). A “+” marks species where Galf has been detected; Galf has not been examined in the other taxa shown. Species where the GLF-encoded UGM activity was confirmed in this work are boxed. For information on the specific sequences analyzed, see Table 1 and Table S2 in the supplemental material. The scale at the bottom corresponds to the fraction of amino acid sequence difference.