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. 2005 Jun;4(6):1116–1124. doi: 10.1128/EC.4.6.1116-1124.2005

FIG. 1.

FIG. 1.

SSY5 and PTR3 can be mutated to activate the AGP1 promoter in the absence of amino acids. (A) Suspensions of strains M5077 (trk1Δ trk2Δ ssy5Δ/pAGP1-KAT1) or M5078 (trk1Δ trk2Δ ptr3Δ/pAGP1-KAT1) transformed with the centromere-based plasmid pRS316 carrying the indicated SSY5 or PTR3 allele or without insert (vector) were spotted onto minimal medium (SD), minimal medium with 0.2 mM leucine (SD + Leu), or minimal medium with 100 mM KCl (SD + KCl) and incubated for 3 days at 30°C. (B) β-Galactosidase activity in cell extracts of strains M5360 (ptr3Δ agp1::Pagp1-lacZ-KanMX) and M5361 (ssy5Δ agp1::Pagp1-lacZ-KanMX) transformed with the centromere-based plasmid pRS316 carrying the indicated PTR3 and SSY5 alleles and grown in minimal medium (SD medium). Standard deviation error bars are shown.