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. 2024 Oct 28;15:9282. doi: 10.1038/s41467-024-53395-y

Fig. 2. CpdR phosphorylation state influences CpdR-PopZ interactions in E. coli.

Fig. 2

a Genes for reconstituting CpdR phosphorylation and PopZ interaction in E. coli. b CpdR-GFP phosphorylation levels in E. coli lysates, observed using Phos-tag gel electrophoresis. The average intensities of the phosphorylated bands as a fraction of the sum of band intensities from three biological replicates are provided, along with the standard deviation between replicates. Replicate gels are provided in Supplementary Fig. 2a. For CckA variants, WT = wildtype; H+ = hyperactive-kinase; K− = kinase-deficient. c mChy-PopZ and CpdR-GFP localization in E. coli cells. Normalized fluorescence intensities were plotted against cell length (n = 60, with 20 cells from 3 biological replicates. Lines trace mean value, shaded regions = SD). Scale bar = 5 μm. d FRAP and FLIP assay for CpdR-GFP in E. coli cells expressing PopZ. Recovery and loss of fluorescence were plotted against time in seconds (n = 20, Lines trace mean value, bar = standard deviation). Scale bar = 2 μm. Source data are provided as a Source Data file.