Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 28;15:9282. doi: 10.1038/s41467-024-53395-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Diagram of CpdR-mediated substrate degradation by ClpXP. Depending on the substrate, an additional adaptor (green) could be RcdA and/or PopA. Graphics: BioRender. b Genetic modifications for observing RcdA -GFP or ClpXP -GFP in different CpdR phosphorylation contexts, built in a ∆cpdR; popZ::mChy-popZ C. crescentus strain background. c Localization of RcdA-GFP or ClpX-GFP and mChy-PopZ in different CpdR phosphorylation contexts. Arrowheads mark polar localization; scale bar = 5 μm. d The average frequencies of cells with diffuse, monopolar, and bipolar fluorescent foci in (c) (n > 100/replicate, bar = standard deviation of 3 biological replicates). Source data and n values for (d) is provided as a Source Data file.