Table 2.

Experimental design: central composite design: 3 factors with 2 responses for SUL/TRIM/INH/B6.

Run	Factor :A	Factor :B	Factor :C	R1: Response1: Resolution (Rs)				R2: Response2 : Asymmetric factor (As)
				R1(SUL)	R1(TRIM)	R1(INH)	R1(B6)	R2(SUL)	R2(TRIM)	R2(INH)	R2(B6)
1	0.95	6.5	13.0454	15.001	28.782	6.322	1.954	1.068	0.954	0.962	1.052
2	0.95	6.5	8	13.111	26.254	7.254	1.641	0.992	0.837	0.730	1.007
3	1.2	7.5	5	13.011	39.329	6.800	1.574	0.965	1.134	0.990	1.307
4	0.95	6.5	8	13.145	26.254	7.254	1.641	0.992	0.835	0.730	1.270
5	0.95	6.5	8	13.185	26.254	6.904	1.522	0.992	0.839	0.730	1.270
6	0.95	6.5	8	13.122	26.254	7.254	1.614	0.992	0.838	0.731	1.270
7	0.95	6.5	2.95462	12.233	29.053	8.52	1.769	0.882	1.086	1.111	1.097
8	1.37045	6.5	8	16.156	35.551	6.176	1.193	1.184	0.822	0.711	1.118
9	0.95	6.5	8	13.155	26.254	7.254	1.854	0.992	0.833	0.732	1.270
10	0.7	7.5	5	12.235	32.060	8.350	1.733	0.993	0.988	0.882	1.076
11	0.7	5.5	5	15.089	17.045	7.556	1.764	0.989	0.921	0.824	1.202
12	0.95	6.5	8	13.185	26.254	7.254	1.726	0.992	0.837	0.737	1.270
13	0.529552	6.5	8	13.322	25.296	7.198	1.936	1.075	0.988	0.884	1.065
14	0.7	7.5	11	15.025	27.083	7.556	1.550	1.302	0.938	0.831	0.892
15	0.95	8.18179	8	10.025	24.43	6.36	1.625	2.079	0.896	0.794	1.020
16	1.2	5.5	11	15.798	28.581	6.045	1.081	0.98	0.901	0.801	1.173
17	0.95	4.81821	8	11.232	19.421	6.34	1.745	1.973	1.019	0.964	0.780
18	1.2	5.5	5	12.252	23.176	6.547	1.543	0.993	0.964	0.867	1.173
19	0.7	5.5	11	14.755	17.845	6.629	1.149	0.865	0.971	0.857	1.108
20	1.2	7.5	11	16.555	33.022	6.557	0.928	1.114	0.926	0.82	1.190

Factor A, Flow Rate of the mobile Phase; Factor B, Mobile phase pH; C, Organic Modifier; R1, Response1, Resolution (Rs), R2, Response2, Asymmetric factor (As); R1(SUL), Resolution of Sulphonamide; R1(TRIM), Resolution of Trimethoprim; R1(INH), Resolution of Isoniazid;R1(B6), Resolution of pyridoxine; R2(SUL), Asymmetric factor of Sulphonamide; R2(TRIM), Asymmetric factor of Trimethoprim; R2(INH), Asymmetric factor of Isoniazid; R2(B6), Asymmetric factor of pyridoxine.