Table 3.
ANOVA coefficients with p-values for response 1(Resolution).
| R1 (SUL) | p-Value | R1 (TRIM) | p-Value | R1 (INH) | p-Value | R1 (B6) | p-Value | |
|---|---|---|---|---|---|---|---|---|
| Intercept | + 13.05 | + 26.25 | + 7.18 | + 1.20 | ||||
| A | + 0.4180 | 0.1744 | + 3.4700 | 0.0005 | −0.4029 | 0.0022 | −0.0171 | 0.0619 |
| B | −0.1970 | 0.5064 | + 3.9052 | 0.0002 | + 0.1557 | 0.1465 | −0.1988 | < 0.0001 |
| C | + 1.1001 | 0.0033 | −0.4053 | 0.5649 | −0.4020 | 0.0023 | −0.2256 | < 0.0001 |
| AB | + 0.5596 | 0.1650 | −0.4574 | 0.6183 | −0.0770 | 0.5644 | + 0.0641 | 0.0006 |
| AC | + 0.5814 | 0.1507 | + 0.4094 | 0.6552 | + 0.0770 | 0.5644 | + 0.0411 | 0.0069 |
| BC | + 0.3881 | 0.3233 | −2.1900 | 0.0338 | + 0.0657 | 0.6218 | + 0.0554 | 0.0014 |
| A2 | + 0.8136 | 0.0152 | + 1.5022 | 0.0470 | −0.0930 | 0.3566 | + 0.0152 | 0.0710 |
| B2 | −0.6139 | 0.0519 | −1.5014 | 0.0467 | −0.3359 | 0.0058 | −0.0869 | 0.0005 |
| C2 | + 0.4424 | 0.1430 | + 0.9687 | 0.1745 | + 0.0176 | 0.8583 | + 0.0571 | < 0.0001 |
Factor A: Flow Rate of the mobile Phase; Factor B: Mobile phase pH; C: Organic Modifier; R1: Response1: Resolution (Rs), R2: Response2: Asymmetric factor (As); R1(SUL): Resolution of Sulphanilamide; R1(TRIM): Resolution of Trimethoprim; R1(INH): Resolution of Isoniazid;R1(B6): Resolution of pyridoxine.