Table 4.
ANOVA coefficients with p-values for Response 2 (Asymmetric Factor).
| R2 (SUL) | p-Value | R2 (TRIM) | p-Value | R2 (INH) | p-Value | R2 (B6) | p-Value | |
|---|---|---|---|---|---|---|---|---|
| Intercept | + 1.00 | + 0.8300 | + 0.7318 | + 1.27 | ||||
| A | + 0.0063 | 0.9164 | −0.0120 | 0.4057 | −0.0143 | 0.4312 | −0.0072 | 0.8534 |
| B | + 0.0531 | 0.3867 | + 0.0014 | 0.9212 | −0.0070 | 0.6964 | + 0.1951 | 0.0077 |
| C | + 0.0464 | 0.4474 | −0.0359 | 0.0266 | −0.0367 | 0.0625 | + 0.2832 | 0.0001 |
| AB | −0.0419 | 0.5969 | + 0.0197 | 0.2994 | + 0.0138 | 0.5607 | + 0.0176 | 0.7485 |
| AC | −0.0061 | 0.9379 | −0.0335 | 0.0931 | −0.0262 | 0.2773 | −0.0739 | 0.2049 |
| BC | + 0.0744 | 0.3549 | −0.0300 | 0.1274 | −0.0238 | 0.3231 | + 0.1931 | 0.0081 |
| A2 | −0.0305 | 0.6047 | + 0.0251 | 0.0917 | + 0.0111 | 0.5295 | −0.0631 | 0.1117 |
| B2 | + 0.2864 | 0.0005 | + 0.0451 | 0.0074 | + 0.0394 | 0.0433 | + 0.0415 | 0.5678 |
| C2 | −0.0852 | 0.1669 | + 0.0672 | 0.0005 | + 0.0965 | 0.0002 | + 0.1607 | 0.0024 |
Factor A, Flow Rate of the mobile Phase; Factor B, Mobile phase pH; C, Organic Modifier; R1, Response1, Resolution (Rs), R2, Response2, Asymmetric factor (As); R2(SUL), Asymmetric factor of Sulphanilamide; R2(TRIM), Asymmetric factor of Trimethoprim; R2(INH), Asymmetric factor of Isoniazid; R2(B6), Asymmetric factor of pyridoxine.