FIG. 4.

Western analysis of the ΔAC mutant containing chsA(p)::HA-chsA. Cell extract from ΔAC/pHBSBXHA-7 [a ΔAC mutant containing chsA(p)::HA-chsA] was fractionated by centrifugation and subjected to Western analysis with anti-HA antibody (α-HA). P10, P100, and S100 indicate the 10,000 × g pellet fraction, 100,000 × g pellet fraction, and 100,000 × g supernatant fraction, respectively. Arrows indicate two major bands of 123 kDa and 146 kDa. In order to show the amount of proteins loaded, 20% of the volume of extract used for Western analysis was subjected to SDS-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue (CBB).