TABLE 1.
A. nidulans strains used in this study
| Strain | Genotypeb | Source |
|---|---|---|
| FGSC A26 | biA1 | FGSCa |
| ABPU/AU | biA1 pyrG89 wA3 argB2 pyroA4 [pSS1][pP1] | 29 |
| A-4 | biA1 pyrG89 wA3 argB2 pyroA4 ΔchsA::argB | 29 |
| C2-11/A-16 | biA1 pyrG89 wA3 argB2 pyroA4 ΔchsC::pyr-4 [pSS1] | 13 |
| AC-7, -8 | biA1 pyrG89 wA3 argB2 pyroA4 ΔchsA::argB ΔchsC::pyr-4 | 13 |
| ABPUS14 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 | This study |
| ///-8, -10 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 [pUCPYR1][pUSC][pSS1] | 56 |
| ABPUS////-7 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 [pUCPYR1][pUSC][pSS1][pUCPYROA] | This study |
| AΔPG/sC-9 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG [pUSC] | 56 |
| AΔPG/sC/A-1 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG [pUSC][pSS1] | 56 |
| AΔPG/sC/A/P-1, -12 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG [pUSC][pSS1] [pUCPYROA] | This study |
| CΔsC/PG/A-1 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsC::sC [pUCPYR1][pSS1] | 56 |
| ΔACa4 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC | 56 |
| ΔAC/A-4 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC [pSS1] | 56 |
| ΔAC/pHBS-4, -5, -6 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsA(p)::chsA::argB | This study |
| ΔAC/pHBSBXHA-7, -8 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsA(p)::HA-chsA::argB | This study |
| AΔPG/sC/pHBSBXHA-2, -3, -9 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG chsA(p)::HA-chsA::argB [pUSC] | This study |
| CΔsC/PG/A/FLAGC-1, -2, -4 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsC::sC chsC(p)::FLAG-chsC::pyroA [pUCPYR1][pSS1] | This study |
| ΔAC/A/P-2, -5, -8 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC [pSS1][pUCPYROA] | This study |
| ΔAC/FLAGC-2, -4 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsC(p)::FLAG-chsC::pyroA | This study |
| ΔAC/A/FLAGC-2, -5 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsC(p)::FLAG-chsC::pyroA [pSS1] | This study |
| ΔAC/ChsC-1, -3 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsC(p)::chsC::pyroA | This study |
| ΔAC/A/ChsC-8, -9, -13 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsC(p)::chsC::pyroA [pSS1] | This study |
| ΔAC/HAA/FLAGC-1, -2 | biA1 pyrG89 wA3 argB2 sC114 pyroA4 ΔchsA::pyrG ΔchsC::sC chsA(p)::HA-chsA::argB chsC(p)::FLAG-chsC::pyroA | This study |
a
Fungal Genetics Stock Center, Kansas City, Kans.
b
The plasmids in brackets were digested with a restriction enzyme that has the sole recognition site on the auxotrophic marker. The linearized plasmid was used for transformation.