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. 2005 Jun;4(6):1057–1065. doi: 10.1128/EC.4.6.1057-1065.2005

FIG. 6.

FIG. 6.

RNase H cleavage by WT and mutant VLPs of an RNA primer containing the PPT sequence used to initiate +sssDNA synthesis. (A) Sequence of the 61-nt DNA template and the −9/+7 RNA primer used in the experiment shown in panel B. The arrow indicates the cleavage site between nt −1 and nt +1 in the RNA primer. The asterisk at the 5′ end of the RNA primer represents the 32P label. (B) The primer template shown in panel A was incubated with WT, Δ2173-3600, and Δ2173-3855 VLPs for 10 min. A control experiment was done with recombinant p6H3601 Ty1 RT at high and low concentrations (conc). Also shown is the result of RNase H cleavage by recombinant p6HEK3838 RT.