Fig 2.

One- and two-dimensional microfluidic cultivation of P. alloputida KT2440 with different Ln elements. One-dimensional screenings have been carried out for La and Er ramping from 0 to 75 nM, over 18 steps (n = 3–5 microfluidic segments per concentration, technical replicates). Per Ln element, two or three tube coils were incubated (n = 2–3, biological replicates). Incubations were done for 72 h. Plotted are normalized autofluorescence units (NFU) and standard deviations at the beginning (black) and end of the incubations (gray) (A). A two-dimensional screening has been done for La vs Er (B). We tested 121 combinations of concentrations (0–75 nM) against each other and incubated the corresponding microfluidic segments for three days. Per combination, between three and five microfluidic segments were generated (technical replicates). Plotted are NFU values and standard deviations after two and three days. For the measurements, complete sequences were moved through the sensors. Especially due to repeated measurements, it can happen that some droplets merge (see supplemental material). For the evaluation, only droplets that have not merged have been considered. Grayed-out tiles refer to droplets that suffered from droplet fusion.