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. Author manuscript; available in PMC: 2024 Oct 29.
Published in final edited form as: Nat Metab. 2024 Feb 27;6(4):697–707. doi: 10.1038/s42255-024-00992-2

Fig. 4: Histone butyrylation on H3K27 is associated with active gene regulatory elements and gene expression in intestinal epithelial cells.

Fig. 4:

(A) Representative tracks of ChIP-seq data in cecal intestinal epithelial cells from female C57BL/6J mice. At least two biological replicates were performed for each ChIP. A representative RNA-seq track is also shown (n = 3). ChIP and RNA signal on a representative housekeeping gene Actb (top) and tributyrin-regulated gene Hk (bottom). (B) Genomic localization of both H3K27ac and H3K27bu consensus peaks of two replicates each and genomic localizations (Genome). X-axis depicts percentage of sites. (C) H3K27bu localizes to highly expressed genes. Meta plots of a representative H3K27bu ChIP signal shown above violin plot displaying the quartiles of gene expression.