Table 4.

Summary of the main characteristics of 16 putative modulators of mutable collagenous tissue (MCT): top BLAST hit, the status of the open reading frame, number of amino acids (Size), molecular weight in Daltons (Weigth), the isoelectric point (pI), the presence of a signal peptide, as predicted by SignalP, and localization of the protein product, as predicted by DeepLoc. Sequences are identified by their suffix name, and all of them have the prefix “TRINITY_.” The corresponding amino acid sequences are available on Zenodo under DOI 10.5281/zenodo.13830943

Sequence	Top BLAST hit	ORF status	Size, aa	Weight (Da)	pI	SignalP	DeepLoc
DN1888_c0_g1	KAF6027037.1 hypothetical protein EB796_014653 (Bugula neritina)	3’ partial	872	87914.35	9.02	No SP	Extracellular
DN9695_c0_g1	XP_038071724.1 uncharacterized protein LOC119740469 (Patiria miniata)	5’ partial	941	105049.37	5.12	SP	Cell Membrane
DN894_c1_g2	XP_022109156.1 uncharacterized protein LOC110989228 (Acanthaster planci)	Complete	574	62324.95	5.25	SP	Extracellular
DN819_c1_g1	No hit	Complete	45	5243.09	4.12	SP	Extracellular
DN81063_c0_g1	XP_022104884.1 uncharacterized protein LOC110986900 (Acanthaster planci)	Internal	525	58241.64	7.18	No SP	Extracellular
DN1190_c0_g1	No hit	Internal	366	36461.54	11.07	No SP	Extracellular
DN722_c0_g2	No hit	Complete	86	9508.15	8.4	SP	Extracellular
DN3456_c0_g2	No hit	3’ partial	109	11956.31	3.74	SP	Extracellular
DN4715_c0_g1	No hit	Complete	176	18570.72	7.35	SP	Cell membrane
DN130227_c0_g1	No hit	3’ partial	47	5507.32	3.91	SP	Extracellular
DN2540_c0_g1	No hit	Complete	451	45656.43	5.69	SP	Extracellular
DN1888_c0_g2	No hit	3’ partial	104	9040.43	10.86	No SP	Nucleus/ extracellular
DN4472_c0_g1	No hit	3’ partial	50	5673.68	4.38	SP	Extracellular
DN24708_c1_g1	No hit	Complete	86	9219.84	8.39	SP	Extracellular
DN2166_c2_g1	No hit	Complete	23	2274.83	8.34	No SP	Extracellular
DN107250_c1_g1	No hit	Complete	65	7394.73	7.56	SP	Extracellular