TABLE. Comparison of sensitivity, limit of detection (LOD), linearity (R2) and efficiency of real-time multiplex and singleplex PCR taqMan assays for detection of Leptospira spp., Rickettsia spp., Borrelia spp., Anaplasma spp., Brucella spp. and Bartonella spp.
| Assay | Target | AT | Singleplex | Multiplex | CV (%) | ||||
| R2 | Efficiency (%) | LOD (DNA copies) | R2 | Efficiency (%) | LOD (DNA copies) | ||||
| 1 | Leptospira spp. | 50ºC | 0.998 | 92.0 | 159.5 | 0.928 | 93.1 | 159.5 | 0.84 |
| Rickettsia spp. | 0.995 | 91.6 | 228.33 | 0.963 | 91.3 | 228.33 | 0.23 | ||
| Borrelia spp. | 0.998 | 94.5 | 22.83 | 0.983 | 94.2 | 22.83 | 0.22 | ||
| 2 | Anaplasma spp. | 60ºC | 0.996 | 93.4 | 22.79 | 0.997 | 94.9 | 227.91 | 1.13 |
| Brucella spp. | 0.993 | 101.8 | 159.5 | 0.923 | 97.6 | 159.5 | 0,87 | ||
| Bartonella spp. | 0.997 | 97.2 | 22.83 | 0.959 | 98.4 | 228.33 | 2.98 | ||
AT: annealing temperature; LOD: limit of detection; R2: coefficient of determination; CV: coefficient of variation.