Fig. 1.

JEV infection significantly increases the permeability of the mouse blood-brain barrier in vivo. (A) JEV-infected mice clinical score. Mice were intraperitoneally injected with 5 × 10^⁴ PFU JEV. For clinical symptom score standards, see Methods details. (B) JEV-infected mice survival curve (mock group: n = 10; JEV group: n = 10). Survival curves were analyzed by Log-rank (Mantel-Cox) test. (C) TCID₅₀ detection of virus titer in JEV-infected mouse brain. TCID₅₀ was analysed by the WilcoxonTest method of non-parametric tests. (D) Schematic diagram of establishing a model for detecting BBB permeability in JEV-infected mice. (E) Picture showing the content of sodium fluorescein in mouse brain. (F) BBB permeability ratio was detected and calculated by measuring sodium fluorescein dye using a microplate reader (n = 3). The data presented represent the mean values ± standard deviations (SD) of three independent experiments with three biological replicates using Student’s t-test. Statistical significance was established at **p < 0.01.The schematic diagram was made with the free version of BioRender