Table 1.

Sequencing data quality control chart by whole genome bisulfite sequencing (WGBS)

Sample ID	Clean Reads Q20 Rate (%)	Clean Data Size (bp)	Mapping Rate (%)	Bisulfite Conversion Rate (%)	Duplication Rate (%)	Average Depth (X)	Coverage (%)
mock1	93.62;94.65	96,122,431,400	91.60	99.59	12.36	23.68	87.322
mock2	93.85;94.83	105,886,943,400	89.97	99.61	6.80	27.65	87.318
mock3	94.42;94.56	96,252,253,600	87.89	99.52	7.56	24.58	87.222
JEV1	94.62;95.18	111,946,471,000	90.73	99.53	7.75	29.54	87.329
JEV2	95.20;95.45	116,417,847,000	90.82	99.56	5.92	31.19	87.363
JEV3	94.70;93.48	98,233,161,800	91.95	99.50	7.64	26.34	87.276

Mapping rate: the proportion of reads that can be mapped to reference genome in total reads

Coverage (%): sequencing coverage of the whole genome