Fig. 5.
Effect of hypoxia-preconditioned MSCs (MSCsHYP, MSCsHYP→APO, MSCsHYP+APO) on the efferocytosis of MSCs and macrophage polarization from proinflammatory (M1) to anti-inflammatory (M2) phenotype. The bar graph represents (A) efferocytosis of MSCs by macrophages (n = 5). (B) the ratio of M1 MФ/M2 MФ in the co-culture of MSCs and M1 MФ (n = 5). Data shown represent the Mean ± S.D of 5 independent experiments performed with PBMNCs derived from 5 different donors (biological replicates), with each experiment conducted in triplicate (technical replicates). Statistical analysis: Tukey’s multiple comparisons test; *≤0.05; **≤0.01; ***≤0.001; ****≤0.0001. Abbreviations: BM: Bone marrow; WJ: Wharton’s Jelly; MSCs: Mesenchymal stem cells; HYP: Hypoxia-preconditioned; APO: Apoptosis; MФ: Macrophages