Figure 1.

Schematics of dual laser illumination for primed conversion. (a) Schematic of the technique originally reported by Dempsey et al. (2015). The author implemented a specially‐designed filter plate in the excitation light path of a CLSM. The filter plate consists of two semi‐circular optical filters transmittable for blue laser or infrared laser with a thick opaque separator in the middle. With this design, the two laser beams can be aligned to meet only in a defined 3D volume, allowing for efficient axial confinement of photoconversion. (b) Schematic of the technique reported in the current study. Samples are simultaneously illuminated by blue and red laser beams in the standard manner used in CLSM. Spatially‐confined primed conversion can be achieved with this simple method.