Gene	Restriction enzyme	Transcription enzyme	References
ndst1	EcoRI	T7	(Mii et al., 2017)
sox3	EcoRI	T3	(Nitta et al., 2006)
foxd3	EcoRI	T7	(Matsukawa et al., 2015)
slug	SpeI	T7	(Matsukawa et al., 2015)
neurod4	PstI	T7	(Matsukawa et al., 2015)
islet1	EcoRI	T7	(Matsukawa et al., 2015)
krt12.4	BamHI	T7	(Watanabe et al., 2018)