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. Author manuscript; available in PMC: 2024 Oct 29.
Published in final edited form as: Methods Enzymol. 2024 Jun 29;703:299–328. doi: 10.1016/bs.mie.2024.05.021

Figure 3.

Figure 3.

SDS page analysis of fractions collected during the purification of 6x Histidine tagged TPARED seen as a single band at 37 kDa. Fractions (5 mL) were collected during the linear elution 0–50% Buffer D over 8 cv. Fractions 32 through 48 were determined to be of sufficient purity (>95%), pooled together, desalted, concentrated, and stored.