Table 3.
Comparison of microarray versus RT-PCR
Ratio* | Pairs† | Percentage‡ | |
Microarray:Taqman | 1:1 | 44 | 55 |
1.5:1 | 4 | 5 | |
2:1 | 3 | 4 | |
> 3:1 | 1 | 1 | |
1:1.5 | 12 | 15 | |
1:2 | 6 | 8 | |
> 1:3 | 10 | 12 | |
Total | 80 | 100 |
*The ratio of expression levels (relative to unchallenged controls) determined by microarray compared to RT-PCR. The ratio of 1:1 is defined as 1:1-1.4. The genes analyzed are listed in Table 2. †Number of microarray:RT-PCR data pairs. Twenty genes were analyzed at four time points, equaling 80 data pairs. ‡Percentage of each category in the total number of data pairs.