Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1983 Aug 15;214(2):293–298. doi: 10.1042/bj2140293

The effects of 24R,25-dihydroxycholecalciferol and of 1 alpha,25-dihydroxycholecalciferol on ornithine decarboxylase activity and on DNA synthesis in the epiphysis and diaphysis of rat bone and in the duodenum.

D Sömjen, I Binderman, Y Weisman
PMCID: PMC1152247  PMID: 6604524

Abstract

The effect of cholecalciferol metabolites on ornithine decarboxylase activity and on DNA synthesis in developing long bones was investigated in vitamin D-depleted rats. In the epiphysis there was a 6.4-fold increase in ornithine decarboxylase activity 5 h after a single injection of 24R,25-dihydroxycholecalciferol but not of 24S,25-dihydroxycholecalciferol or other vitamin D metabolites. In comparison, in the diaphysis and duodenum, 1 alpha,25-dihydroxycholecalciferol, but not other vitamin D metabolites, caused a 3-3.5-fold increase in the enzyme activity. The enzyme activity in the tissues examined attained a maximal value at 5 h after the injection of the metabolites. The activity of ornithine decarboxylase in the epiphysial region increased dose-dependently as the result of a single injection of 24R,25-dihydroxycholecalciferol and attained a maximal value at a dose between 30 and 3000 ng. In addition, administration of 24R,25-dihydroxycholecalciferol, but not 24S,25-dihydroxycholecalciferol or other metabolites, caused within 24 h a 1.7-2.0-fold increase in [3H]thymidine incorporation into DNA of the epiphyses of tibial bones. In comparison, 1 alpha,25-dihydroxycholecalciferol caused a 1.5-fold increase in [3H]thymidine incorporation into DNA of the diaphyses and of the duodenum. The present data indicate that 24R,25-dihydroxycholecalciferol is involved in the regulation of epiphyseal growth, whereas 1 alpha,25,dihydroxycholecalciferol stimulates the proliferation of cells in the diaphysis of long bones and in the intestinal mucosa.

Full text

PDF
293

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brumbaugh P. F., Haussler M. R. 1 Alpha,25-dihydroxycholecalciferol receptors in intestine. II. Temperature-dependent transfer of the hormone to chromatin via a specific cytosol receptor. J Biol Chem. 1974 Feb 25;249(4):1258–1262. [PubMed] [Google Scholar]
  3. Cohen S., O'Malley B. W., Stastny M. Estrogenic induction of ornithine decarboxylase in vivo and in vitro. Science. 1970 Oct 16;170(3955):336–338. doi: 10.1126/science.170.3955.336. [DOI] [PubMed] [Google Scholar]
  4. Corvol M., Ulmann A., Garabedian M. Specific nuclear uptake of 24,25-dihydroxycholecalciferol, a vitamin D3 metabolite biologically active in cartilage. FEBS Lett. 1980 Jul 28;116(2):273–276. doi: 10.1016/0014-5793(80)80661-2. [DOI] [PubMed] [Google Scholar]
  5. Durham A. C., Walton J. M. Calcium ions and the control of proliferation in normal and cancer cells. Biosci Rep. 1982 Jan;2(1):15–30. doi: 10.1007/BF01142195. [DOI] [PubMed] [Google Scholar]
  6. Eilam Y., Szydel N., Harell A. Effects of vitamin D metabolites on cellular Ca2+ and on Ca transport in primary cultures of bone cells. Mol Cell Endocrinol. 1980 Sep;19(3):263–273. doi: 10.1016/0303-7207(80)90056-8. [DOI] [PubMed] [Google Scholar]
  7. Eisman J. A., Hamstra A. J., Kream B. E., DeLuca H. F. 1,25-Dihydroxyvitamin D in biological fluids: a simplified and sensitive assay. Science. 1976 Sep 10;193(4257):1021–1023. doi: 10.1126/science.1085035. [DOI] [PubMed] [Google Scholar]
  8. Endo H., Kiyoki M., Kawashima K., Naruchi T., Hashimoto Y. Vitamin D3 metabolites and PTH synergistically stimulate bone formation of chick embryonic femur in vitro. Nature. 1980 Jul 17;286(5770):262–264. doi: 10.1038/286262a0. [DOI] [PubMed] [Google Scholar]
  9. Fukuda S., Kopple J. D. Evidence that dog kidney is an endogenous source of histidine. Am J Physiol. 1979 Jul;237(1):E1–E5. doi: 10.1152/ajpendo.1979.237.1.E1. [DOI] [PubMed] [Google Scholar]
  10. Haussler M. R., McCain T. A. Basic and clinical concepts related to vitamin D metabolism and action (first of two parts). N Engl J Med. 1977 Nov 3;297(18):974–983. doi: 10.1056/NEJM197711032971804. [DOI] [PubMed] [Google Scholar]
  11. Holick M. F., Garabedian M., DeLuca H. F. 1,25-dihydroxycholecalciferol: metabolite of vitamin D3 active on bone in anephric rats. Science. 1972 Jun 9;176(4039):1146–1147. doi: 10.1126/science.176.4039.1146. [DOI] [PubMed] [Google Scholar]
  12. Holick M. F., Kleiner-Bossaller A., Schnoes H. K., Kasten P. M., Boyle I. T., DeLuca H. F. 1,24,25-Trihydroxyvitamin D3. A metabolite of vitamin D3 effective on intestine. J Biol Chem. 1973 Oct 10;248(19):6691–6696. [PubMed] [Google Scholar]
  13. Hölttä E., Jänne J. Ornithine decarboxylase activity and the accumulation of putrescine at early stages of liver regeneration. FEBS Lett. 1972 Jun 1;23(1):117–121. doi: 10.1016/0014-5793(72)80298-9. [DOI] [PubMed] [Google Scholar]
  14. Icekson I., Kaye A. M. In vitro inactivation of ornithine decarboxylase by a heat-labile factor from rat ventral prostate. FEBS Lett. 1976 Jan 1;61(1):54–58. doi: 10.1016/0014-5793(76)80170-6. [DOI] [PubMed] [Google Scholar]
  15. Kaye A. M., Icekson I., Lamprecht S. A., Gruss R., Tsafriri A., Lindner H. R. Stimulation of ornithine decarboxylase activity by luteinizing hormone in immature and adult rat ovaries. Biochemistry. 1973 Jul 31;12(16):3072–3076. doi: 10.1021/bi00740a020. [DOI] [PubMed] [Google Scholar]
  16. Kaye A. M., Icekson I., Lindner H. R. Stimulation by estrogens of ornithine and S-adenosylmethionine decarboxylases in the immature rat uterus. Biochim Biophys Acta. 1971 Oct;252(1):150–159. doi: 10.1016/0304-4165(71)90103-6. [DOI] [PubMed] [Google Scholar]
  17. Lawson D. E., Wilson P. W., Kodicek E. Metabolism of vitamin D. A new cholecalciferol metabolite, involving loss of hydrogen at C-1, in chick intestinal nuclei. Biochem J. 1969 Nov;115(2):269–277. doi: 10.1042/bj1150269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. O'Malley B. W., Schrader W. T. The receptors of steroid hormones. Sci Am. 1976 Feb;234(2):32–43. doi: 10.1038/scientificamerican0276-32. [DOI] [PubMed] [Google Scholar]
  19. Ornoy A., Goodwin D., Noff D., Edelstein S. 24, 25-dihydroxyvitamin D is a metabolite of vitamin D essential for bone formation. Nature. 1978 Nov 30;276(5687):517–519. doi: 10.1038/276517a0. [DOI] [PubMed] [Google Scholar]
  20. Pegg A. E., Williams-Ashman H. G. Biosynthesis of putrescine in the prostate gland of the rat. Biochem J. 1968 Jul;108(4):533–539. doi: 10.1042/bj1080533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reach G., Elkik F., Parry C., Corvol P., Milleiz P. Increased urate excretion after o,p'-DDD. Lancet. 1978 Jun 10;1(8076):1269–1269. doi: 10.1016/s0140-6736(78)92515-1. [DOI] [PubMed] [Google Scholar]
  22. Russell D., Snyder S. H. Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1420–1427. doi: 10.1073/pnas.60.4.1420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shinki T., Takahashi N., Miyaura C., Samejima K., Nishii Y., Suda T. Ornithine decarboxylase activity in chick duodenum induced by 1 alpha, 25-dihydroxycholecalciferol. Biochem J. 1981 Jun 1;195(3):685–690. doi: 10.1042/bj1950685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Somjen D., Binderman I., Berger E., Harell A. Bone remodelling induced by physical stress is prostaglandin E2 mediated. Biochim Biophys Acta. 1980 Jan 3;627(1):91–100. doi: 10.1016/0304-4165(80)90126-9. [DOI] [PubMed] [Google Scholar]
  25. Spencer R., Charman M., Lawson D. E., Emtage J. S. Production and properties of vitamin-D-induced mRNA for chick calcium-binding protein. Eur J Biochem. 1976 Dec 11;71(2):399–409. doi: 10.1111/j.1432-1033.1976.tb11127.x. [DOI] [PubMed] [Google Scholar]
  26. Sömjen D., Sömjen G. J., Harell A., Mechanic G. L., Binderman I. Partial characterization of a specific high affinity binding macromolecule for 24R,25 dihydroxyvitamin D3 in differentiating skeletal mesenchyme. Biochem Biophys Res Commun. 1982 May 31;106(2):644–651. doi: 10.1016/0006-291x(82)91159-7. [DOI] [PubMed] [Google Scholar]
  27. Sömjen D., Sömjen G. J., Weisman Y., Binderman I. Evidence for 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats. Biochem J. 1982 Apr 15;204(1):31–36. doi: 10.1042/bj2040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Takigawa M., Watanabe R., Ishida H., Asada A., Suzuki F. Induction by parathyroid hormone of ornithine decarboxylase in rabbit costal chondrocytes in culture. J Biochem. 1979 Jan;85(1):311–314. doi: 10.1093/oxfordjournals.jbchem.a132326. [DOI] [PubMed] [Google Scholar]
  29. Wasserman R. H., Corradino R. A., Taylor A. N. Vitamin D-dependent calcium-binding protein. Purification and some properties. J Biol Chem. 1968 Jul 25;243(14):3978–3986. [PubMed] [Google Scholar]
  30. Weisman Y., Reiter E., Rott A. Measurement of 24,25 dihydroxyvitamin D in sera of neonates and children. J Pediatr. 1977 Dec;91(6):904–908. doi: 10.1016/s0022-3476(77)80887-1. [DOI] [PubMed] [Google Scholar]
  31. Zerwekh J. E., Haussler M. R., Lindell T. J. Rapid enhancement of chick intestinal DNA-dependent RNA polymerase II activity by 1 alpha, 25-dihydroxyvitamin D3, in vivo. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2337–2341. doi: 10.1073/pnas.71.6.2337. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES