Fig. 6.

The DNA-binding domain of A. thaliana and Brassica RRBs shows a high level of amino acid conservation. Domain structure of A. thaliana and Brassica RRBs and alignment of the amino acid sequences of the GARP-like DNA-binding domain for the selected RRBs from A. thaliana and assayed Brassica species. Conserved amino acids are highlighted, and the percentage identity is shown. The CLUSTAL color scheme was used to color the alignment, reflecting the physicochemical properties of amino acids (Kunzmann et al., 2020). The asterisk denotes the ARR10 residues proposed to interact directly with DNA (Hosoda et al., 2002); for a comprehensive list of RRB alignments, refer to Supplementary Fig. S5.