Fig. 8.

The Arabidopsis TCSv2:3×VENUS cytokinin reporter (Steiner et al., 2020) is cytokinin responsive in B. rapa and B. napus. (A) Scheme of the TCSv2:3×VENUS (after Steiner et al., 2020). (B) Comparison of the relative fluorescence intensity of the TCSv2:3×VENUS cytokinin reporter in BAP-treated hairy roots of B. rapa and B. napus at different time points (0.5, 1, 2, and 4 h) of cytokinin (5 µM BAP) treatment. Means ±SE are shown in the plots. Asterisks indicate statistical significance (***P<0.001, **P<0.01, and *P<0.05, Dunnett’s test). (C) Representative images of B. rapa and B. napus hairy root tips treated with DMSO and BAP throughout the treatment period, showing the measured fluorescent signal intensities in a single root (top) and the corresponding image analyzed by IMARIS software (below). Scale bars represent 40 µm.