Figure 3.

USP36 deubiquitinates Las1L and Nol9 and regulates their levels. A and B, Knockdown of USP36 reduces the levels of Las1L and Nol9. The HEK293 (A) and HeLa (B) cells transfected with scrambled (scr) or USP36 siRNA were assayed by IB. C, The stability of Las1L and Nol9 is dependent on each other. HeLa and HEK293 cells transfected with scr, Las1L, or Nol9 siRNA were assayed by IB. D, USP36 deubiquitinates Las1L. H1299 cells transfected with the indicated plasmids were subjected to Ni²⁺-NTA PD under denaturing conditions, followed by IB to detect the ubiquitinated species of Las1L. The protein expression is shown in the bottom panels. E, Knockdown of endogenous USP36 increases the ubiquitinated species of Las1L. H1299 cells transfected with the indicated plasmids together with scr or USP36 siRNA were subjected to Ni²⁺-NTA PD under denaturing conditions, followed by IB to detect the ubiquitinated species of Las1L. The protein expression is shown in the bottom panels. F, USP36 deubiquitinates Nol9. H1299 cells transfected with the indicated plasmids were subjected to Ni²⁺-NTA PD under denaturing conditions, followed by IB to detect the ubiquitinated species of Nol9. The protein expression is shown in the bottom panels.