Figure 6.

The Las1L–Nol9 complex and USP36 play a role in rRNA ITS2 processing. The HEK293 and HeLa cells transfected with scr, Las1L, Nol9 siRNA (A and B), or USP36 siRNA (C and D) were assayed for rRNA processing by Northern blot analysis using probe hybridizing to the ITS2 (A and C). The 47S pre-rRNA, 32S rRNA, 12S rRNA, and the relative ratios of 32S to 28S rRNA normalized to the scr control are shown in the top panels. Ethidium bromide (EB) staining of the RNA gels to indicate 28S and 18S rRNAs is shown in the bottom panels (A and C). The protein expression is shown by IB (B and D).