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. 2024 Oct 30;4(10):2835–2845. doi: 10.1158/2767-9764.CRC-24-0312

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©2024 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

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Las1L SUMOylation at K565 is critical for its function in rRNA ITS2 processing. The HEK293 (A and B) and HeLa (C and D) cells transfected with scr or Las1L siRNA together with siRNA-resistant WT Flag-Las1L or its K565R mutant were assayed for rRNA ITS2 processing by Northern blot analysis using the ITS2 probe. The 32S rRNA precursor and the relative ratios of 32S to 28S rRNA normalized to the scr control are indicated in the top panels. Ethidium bromide (EB) staining of the RNA agarose gel is shown in the bottom panels (A and C). One representative experiment from three independent experiments is shown. The expression of the endogenous Las1L and exogenous Flag-Las1L is shown by IB (B and D).