Figure 6.

ROS inhibition alleviates senescence in IL-1β-treated SFZCs. SFZCs were treated with IL-1β (20 ng/ml) and (or) NAC (2 μM) for 5 days. (A) SA-β-gal staining and quantitative analysis of SA-β-gal positive cells. (B) Western blot analysis of senescence marker p53. (C and D) mRNA Expression levels of SASP factors Mmp-3 and Mmp-13 were determined using RT-qPCR. ROS = reactive oxygen species; IL-1β = interleukin-1β; SFZCs = superficial zone cells in articular cartilage; NAC = N-acetylcysteine; SA-β-gal = senescence-associated β-galactosidase; mRNA = messenger RNA; SASP = senescence-associated secretory phenotype; RT-qPCR = reverse transcription-quantitative PCR; GAPDH = glyceraldehyde-3-phosphate dehydrogenase. *P < 0.05 and **P < 0.01.