Table 2.

Repeatability of the quadruplex real-time quantitative PCR assay.

Standard plasmid	Concentration of template (copies/μL)	Intra-coefficient of variation		Inter-coefficient of variation
		X ± SD	CV (%)	X ± SD	CV (%)
pMD- TTSuV1	107	18.215 ± 0.052	0.28	18.302 ± 0.026	0.14
	105	24.168 ± 0.120	0.50	24.621 ± 0.106	0.43
	103	31.027 ± 0.251	0.81	31.105 ± 0.351	1.13
pMD- PCV2	107	14.315 ± 0.162	1.13	14.410 ± 0.101	0.70
	105	21.138 ± 0.111	0.53	21.056 ± 0.245	1.16
	103	27.866 ± 0.316	1.13	27.541 ± 0.277	1.01
pMD- PRV	107	12.715 ± 0.182	1.43	12.605 ± 0.113	0.90
	105	19.308 ± 0.189	0.98	19.425 ± 0.203	0.43
	103	25.899 ± 0.213	0.82	26.014 ± 0.104	0.40
pMD-PPV	107	16.383 ± 0.122	0.74	16.162 ± 0.053	0.33
	105	23.107 ± 0.108	0.47	23.216 ± 0.123	0.53
	103	29.851 ± 0.253	0.85	29.546 ± 0.364	1.23