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. 2023 Jul 15;50(6):971–992. doi: 10.1080/1040841X.2023.2223704

Table 1.

Data extraction and study characteristics of selected articles: molecular classes analyzed in bacterial biofilms, sorted by an imaging technique.

Author, year Imaging modality Molecular class imaged
MSI [15]
 Davies et al. (2017) SIMS Quorum-sensing molecules
 Dunham et al. (2018) SIMS Quorum-sensing molecules
 Ding et al. (2016) SIMS (liquid) Quorum-sensing molecules, fatty acids, lipids, carbohydrates
 Hua et al. (2014) SIMS (liquid) Fatty acids
 Hua et al. (2015) SIMS (liquid) Fatty acids
 Zhou et al. (2016) SIMS (liquid) Molecular fragments (non-specified)
 Blaze et al. (2012) MALDI Proteins, peptides
 Brockmann et al. (2019) MALDI-2 Quorum-sensing molecules, metabolites
 Brockmann et al. (2021) IR-MALDI-2 Quorum-sensing molecules, lipids
 Lukowski et al. (2021) MetA-LDI Proteins, peptides, lipids
 Kurczy et al. (2015) NIMS Molecular fragments (non-specified)
 Louie et al. (2013) NIMS Metabolites
 Cui et al. (2013) Fs-LDPI-MS Metabolites
 Zhang et al. (2020) Cryo-OrbiSIMS Lipids, quorum-sensing molecules, nucleobases, metabolites
 Watrous et al. (2013) nanoDESI Proteins, peptides, lipids, fatty acids
Spectroscopy [7]
 Bodelón et al. (2016) SERS Metabolites
 Polisetti et al. (2017) SERS Metabolites
 Do et al. (2019) SERS Metabolites
 Baig et al. (2016) SERS Metabolites, quorum-sensing molecules
 Ivleva et al. (2010) SERS Metabolites, proteins, carbohydrates
 Garg et al. (2022) SERS Proteins, carbohydrates, DNA
 Holman et al. (2009) SR-FTIR Proteins, carbohydrates, DNA
Fluorescence imaging [4]
 Berk et al. (2012) CLSM Proteins, carbohydrates
 Decker et al. (2015) CLSM Proteins
 Narasimhan et al. (2017) CLSM Carbohydrates
 El-Kirat-Chatel et al. (2014) Fluorescence microscopy Proteins
NMR [1]
 Simkins et al. (2018) NMR Oxygen
µCT [1]
 Keren-Paz et al. (2018) µCT Calcium carbonate
Multimodal [5]
 Lanni et al. (2014) SIMS, MALDI Metabolites, quorum-sensing molecules
 Lanni et al. (2014) SIMS, CRM Quorum-sensing molecules, proteins, carbohydrates
 Baig et al. (2015) SIMS, CRM Quorum-sensing molecules
 Dunham et al. (2016) SIMS, MetA-SIMS, MetA-LDI Quorum-sensing molecules
 Si et al. (2016) MALDI, fluorescence microscopy Metabolites