Fig. 3. Inhibition of Notch, Notch-regulated cytokines, or PD1 reduces lung metastases.

Representative histological images of metastatic foci in lungs of 4T1 tumor–bearing mice at the end of stage 2 treatment with (A) vehicle control, (B) LY411575, (C) anti-PD1, and (D) LY411575 f/b anti-PD1. (E to G) Normalized size of lung metastasis following monotherapy or sequential treatment (n = 3 mice per group). (H to J) Representative images of anti-CD68–stained lung after stage 2 of control, anakinra, or sequentially treated animals. (K) CD68⁺ infiltrates in metastases and adjacent lung following treatment (normalized to the number of CD68⁺ macrophages per mm² of control lung; n ≥ 8 quantified regions from 3 mice per group). (L to N) Representative images of anti-CD8–stained lung after stage 2. (O) CD8⁺ infiltrates in metastases and adjacent lung following treatment (normalized to the number of CD8⁺ cells per mm² of control lung; n ≥ 8 quantified regions from 3 mice per group). Bar graphs represent mean + SEM. Bloxplots represent median ± IQR, whiskers indicate 1.5 × IQR. *P < 0.05.