Figure 3.

Enzyme activity of LcDXR. (a) SDS-PAGE analysis of recombination protein with His tag. M: Protein Marker, 1: Insoluble protein before purified, 2: Cell lysate supernatant, 3: Transmission fluid, 4: Washing fluid, 5−8: Elution fluid with protein. (b) Western blot analysis of recombinant LcDXR protein. M: Protein Marker, 1: The inclusion protein, 2: Total protein induced in pET–LcDXR2/Arctic Express, 3: Washing fluid. (c) Enzyme activity assay of recombinant LcDXR. NADPH has a robust ultraviolet absorption peak at A340 and NADP+ does not, while the light absorption value of NADPH at A340 decreases during the catalytic reaction. Data represent the mean ± SEs of three biological replicates.