Table 1. Levels of globin mRNAs in control and hemin treated K562 cells: pooled and single cell determinations.
amol globin mRNA/µg total RNA | mean amol globin mRNA/cell | |||
---|---|---|---|---|
Globin message | Control | Hemin treated | Control | Hemin treated |
α-globin |
1100 ± 110 |
2400 ± 230 |
0.017 ± 0.021 (1 × 104)a |
0.052 ± 0.058 (3.1 × 104) |
γ-globin |
6300 ± 660 |
9300 ± 480 |
1.4 ± 1.6 (8.4 × 105) |
3.7 ± 2.4 (2.2 × 106) |
ɛ-globin | 120 ± 6 | 270 ± 16 | 0.0015 ± 0.0025 (9 × 102) | 0.0062 ± 0.0046 (3.7 × 103) |
aCopy number.