Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody |
β-Actin (mouse monoclonal) |
Proteintech | 66009 RRID:AB_2883475 |
1:10,000 |
| Antibody | GAPDH (mouse monoclonal) |
Absin | Abs830030ss RRID:AB_2811228 |
1:50,000 |
| Antibody |
β-Tubulin (mouse monoclonal) |
Fdbio Science | FD0064 RRID:AB_3076327 |
1:10,000 |
| Antibody | Lamin B (mouse monoclonal) |
Proteintech | 66095 RRID:AB_2721256 |
1:10,000 |
| Antibody | Claudin-5 (rabbit polyclonal) |
Wanleibio | WL03731 RRID:AB_3076320 |
1:1000 |
| Antibody | Occludin (rabbit polyclonal) |
Wanleibio | WL01996 RRID:AB_3076325 |
1:500 |
| Antibody | ZO-1 (mouse polyclonal) |
Proteintech | 21773-1-AP RRID:AB_10733242 |
1:5000 |
| Antibody | VE-cadherin (rabbit polyclonal) |
Wanleibio | WL02033 RRID:AB_3076321 |
1:1000 |
| Antibody |
β-Catenin (rabbit polyclonal) |
Wanleibio | WL0962a RRID:AB_3076323 |
1:5000 |
| Antibody | BCPR (rabbit polyclonal) |
CST | 4477S RRID:AB_10544928 |
1:1000 |
| Antibody | P-gp (rabbit monoclonal) |
CST | 13978S RRID:AB_2798357 |
1:1500 |
| Antibody | p-AKT (mouse monoclonal) |
Huaan Biotechnology | ET1607 RRID:AB_2940863 |
1:2000 |
| Antibody | AKT (mouse monoclonal) |
Huaan Biotechnology | ET1609 RRID:AB_3069857 |
1:2000 |
| Antibody | p-ERK (rabbit polyclonal) |
Proteintech | 28733-1-AP RRID:AB_2881202 |
1:1000 |
| Antibody | ERK (rabbit polyclonal) |
Proteintech | 11257-1-AP RRID:AB_2139822 |
1:1000 |
| Antibody | p-p38 (rabbit monoclonal) |
CST | 4511S RRID:AB_10890701 |
1:250 |
| Antibody | p38 (rabbit monoclonal) |
CST | 8690S RRID:AB_10999090 |
1:250 |
| Antibody | p-JNK (rabbit polyclonal) |
Wanleibio | WL01813 RRID:AB_2910628 |
1:1000 |
| Antibody | JNK (rabbit polyclonal) |
Wanleibio | WL01295 RRID:AB_3064853 |
1:1000 |
| Antibody | FOXO1 (rabbit polyclonal) |
Proteintech | 18592 RRID:AB_2934932 |
1:1000 |
| Antibody | p-FOXO1 (rabbit polyclonal) |
Wanleibio | WL03634 RRID: AB_3076326 |
1:1000 |
| Antibody | ETS1 (mouse monoclonal) |
Santa Cruz | sc-55581 RRID:AB_831289 |
1:500 |
| Antibody | ETS1 (mouse monoclonal) |
Proteintech | 66598 RRID:AB_2881958 |
1:3000 |
| Cell line (Homo sapiens) | hCMEC/D3 cells | JENNIO Biological Technology, Guangzhou, China |
Cat#JNO-H0520 RRID:CVCL_U985 |
Authenticated (STR profiling) |
| Cell line (H. sapiens) | U251 cells | Cellcook Biological Technology, Guangzhou, China | Cat#CC1701 RRID:CVCL_0021 |
Authenticated (STR profiling) |
| Cell line (H. sapiens) | SH-SY5Y cells | Cellcook Biological Technology, Guangzhou, China | Cat#CC2101 RRID:CVCL_0019 |
Authenticated (STR profiling) |
| Software, algorithm | GraphPad Prism | Version 8.0.2 | RRID:SCR_002798 | |
| Software, algorithm | BioTek Cytation 5 Cell Imaging Multi-Mode Reader | BioTek Cytation 5 | RRID:SCR_019732 | |
| Software, algorithm | QuantStudio 3 Real Time PCR System | QuantStudio 3 | RRID:SCR_018712 | |
| Software, algorithm | FACS Celesta Flow Cytometer | BD Biosciences | RRID:SCR_019597 | |
| Software, algorithm | Flowjo software | Version 10.4 | RRID:SCR_008520 | |
| Commercial assay or kit | GDNF-Elisa kit | R&D system RRID:SCR_006140 |
Cat#212-GD | |
| Commercial assay or kit | bFGF-Elisa kit | Elabscience RRID:SCR_025982 |
Cat#E-EL-H6042 | |
| Commercial assay or kit | IGF-1-Elisa kit | Elabscience RRID:SCR_025982 |
Cat#E-EL-H0086 | |
| Commercial assay or kit | TGF-β-Elisa kit | Elabscience RRID:SCR_025982 |
Cat#E-EL-0162 | |
| Peptide, recombinant protein | GDNF | R&D system RRID:SCR_006140 |
Cat#212-GD | |
| Peptide, recombinant protein | bFGF | MedChemExpress RRID:SCR_025062 |
Cat#HY-P7331 | |
| Peptide, recombinant protein | IGF-1 | MedChemExpress RRID:SCR_025062 |
Cat#HY-P70783 | |
| Peptide, recombinant protein | TGF-β | MedChemExpress RRID:SCR_025062 |
Cat#HY-P70543 | |
| Chemical compound, drug | SPP-86 | MedChemExpress RRID:SCR_025062 |
Cat#HY-110193 | |
| Chemical compound, drug | PP2 | MedChemExpress RRID:SCR_025062 |
Cat#HY-13805 | |
| Chemical compound, drug | LY294002 | MedChemExpress RRID:SCR_025062 |
Cat#HY-10108 | |
| Chemical compound, drug | U0126 | MedChemExpress RRID:SCR_025062 |
Cat#S1102 | |
| Chemical compound, drug | SP600125 | Selleck RRID:SCR_003823 |
Cat#HY-12041 | |
| Chemical compound, drug | SB203580 | MedChemExpress RRID:SCR_025062 |
Cat#HY-10256 |