Figure 8. PCR-based genotyping.

(A) List of primers used for routine genotyping. PCR was performed using genomic DNA from mouse tail biopsies. (B) Genotyping of Slc30a1-3xflag-EGFP (Slc30a1^flag-EGFP/+) mouse line. The PCR fragment length for the wild-type (WT) Slc30a1 allele is 612 and 469 bp for the mutant allele, respectively. (C) The conditional knockout (Slc30a1^fl/fl;Lyz2-Cre) mice were recognized by genomic PCR rendering one band of flox/flox allele (512 bp) with two bands of Lyz2-Cre heterozygous (410 and 340 bp). Each lane represents one individual mice.