Figure 7.

Validation of select genes expressed in zebrafish and mouse HCs.(A) Quantified expression of select genes in mouse IHCs and OHCs, and zebrafish HCs and SCs based on published RNA-seq data (9, 10). (B) Confirmed expression of select genes in mouse IHCs, OHCs, stria vascularis (SV), as well as zebrafish HCs and SCs using RT-PCR. (C) Molecular expression of select gene orthologs visualized using smFISH in mouse (left) and zebrafish (right) inner ear tissues (Target probe: red; DAPI: blue). (D) Expression of slc17a8 and cacnb3b in zHCs detected by smFISH. (E) Confocal images showing expression of SLC7A14 in mouse IHCs (top panel) and Slc7a14 in zHCs (bottom panel). Scale bar [applied to all images in (C–E)]: 10 μm.