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. 2024 Oct 17;15:1469180. doi: 10.3389/fphar.2024.1469180

FIGURE 2.

FIGURE 2

β-Elemene treatment can induce ferroptosis in Radioresistant GC cells. (A–B) MKN-45/IR and AGS/IR cells were treated for 48 h with 4 Gy irradiation and/or β-elemene (100 mg/L) combined with various cell death inhibitors, followed by cell viability assessment using the CCK-8 assay. (C–D) The levels of reactive oxygen species (ROS) in MKN-45/IR and AGS/IR cells were measured 48 h after treatment with β-elemene (100 mg/L) and/or irradiation (4 Gy) using flow cytometry. (E–F) The intracellular levels of Fe2+ in MKN-45/IR and AGS/IR cells were determined 48 h post-treatment with β-elemene (100 mg/L) and/or radiation (4 Gy) using an Fe2+ detection assay kit. (G–H) The levels of malondialdehyde (MDA) in MKN-45/IR and AGS/IR cells were analyzed 48 h after treatment with β-elemene (100 mg/L) and/or various doses of radiation. (I–L) The expression of GPX4 in AGS/IR and MKN-45/IR cells was evaluated 48 h post-treatment with β-elemene (100 mg/L) and/or radiation (4 Gy) using qRT-PCR and Western blot. **p < 0.01, ***p < 0.001.