FIGURE 3.

β-Elemene promotes the ubiquitination and degradation of GPX4 in Radioresistant GC cells. (A–F) Western blot analysis of GPX4 protein levels in MKN-45/IR and AGS/IR cells treated with radiation (4 Gy) with or without β-elemene (100 mg/L). Cells were harvested at 0, 2, 4, and 6 h after treatment with CHX. (G–J) Western blot analysis of GPX4 protein levels in MKN-45/IR and AGS/IR cells treated with radiation (4 Gy) with or without β-elemene (100 mg/L). Cells were collected at 0, 2, 4, and 6 h after treatment with either MG-132 or CQ. (K–L) Investigation of ubiquitination levels of GPX4 in cells co-transfected with Ub plasmid, Flag-GPX4, and MG132, treated with radiation (4 Gy) with or without β-elemene (100 mg/L).