Figure 2.

Lipolysis inhibition ameliorates COPD pathogenesis in chronic CS-exposed mice

(A) Experimental scheme for (B)–(J).

(B) Immunoblot showing ATGL expression in GAT of the indicated groups of mice (n = 4 mice per group, representative one of 3 technical replicates).

(C–F) Cell count analysis of total cells, neutrophils, macrophages, and lymphocytes in BALF of the indicated groups of mice (n = 5 mice per group).

(G) Representative images of Masson’s trichrome staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(H) Representative images of H&E staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(I) Mean linear intercept of alveolus of the indicated groups of mice (n = 5 mice per group).

(J) Alveolar area of the indicated groups of mice (n = 5 mice per group).

(K) Experimental scheme for (L)–(R).

(L) Representative images of Masson’s trichrome staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(M) Representative images of H&E staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(N) Mean linear intercept of alveolus of the indicated groups of mice (n = 5 mice per group).

(O) Alveolar area of the indicated groups of mice (n = 5 mice per group).

(P) Body weight of the indicated groups of mice (n = 5 mice per group).

(Q) Relative GAT weight of the indicated groups of mice (n = 5 mice per group).

(R) Relative FFA levels in serum of the indicated groups of mice (n = 5 mice per group). The number of biological replicates is indicated (each dot and immunoblot lane in graphs indicate data from individual mice); error bars denote mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 (t test).