Figure 6.

Therapeutic targeting of CD36 attenuates lung pathogenesis and promotes lung regeneration

(A) Experimental scheme for (B)–(I).

(B) Representative images of Masson’s trichrome staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(C) Representative images of H&E staining of lung of the indicated groups (scale bars, 50 μm, n = 5 mice per group).

(D) Mean linear intercept of alveolus of the indicated groups of mice (n = 5 mice per group).

(E) Alveolar area of the indicated groups of mice (n = 5 mice per group).

(F–H) Immunoblot showing CD36 (F), AXIN2 and β-catenin (G), and ZBP1, p-MLKL, and MLKL (H) expression in lung epithelial cells of the indicated groups of mice (n = 4–5 mice per group, representative one of 3 technical replicates).

(I) qRT-PCR analysis showing Sftpc and T1α mRNA expression in lung tissue of the indicated groups of mice (n = 5 mice per group, mean value of 3 technical replicates).

(J) Experimental scheme for (K)–(O).

(K) Representative images of H&E staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(L) Mean linear intercept of alveolus of the indicated groups of mice (n = 5 mice per group).

(M) Alveolar area of the indicated groups (n = 5 mice per group).

(N) Representative images of Masson’s trichrome staining of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group).

(O) Representative immunofluorescent images of SFTPC (red) and DAPI (blue) of lung of the indicated groups of mice (scale bars, 50 μm, n = 5 mice per group). The number of biological replicates is indicated (each dot and immunoblot lane in graphs indicate data from individual mice); error bars denote mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01 (t test).

See also Figure S5.