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. 2024 Sep 17;5(9):101734. doi: 10.1016/j.xcrm.2024.101734

Figure 3.

Figure 3

Patients with S. aureus bacteremia display reduced WTA-specific IgM antibody levels

(A and B) Normalized binding of (A) IgG2 and (B) IgM to beads coated with TarS-WTA, TarP-WTA, and TarM-WTA. Boxplots represent data for healthy donors (HD, n = 31) and ICU patients with S. aureus (SA, n = 36) or S. pyogenes (GAS, n = 13) bacteremia and extend from the 25th to 75th percentiles. The line inside the box represents the median, whiskers indicate the total range with symbols representing individual donors. Statistical analysis was performed using a Kruskal-Wallis test with Dunn’s multiple comparison test to compare both patient cohorts with healthy donors.

(C) IgG and IgM binding to S. aureus strain Newman Δspa/sbi in sera from healthy donors (n = 11) and patients with S. aureus infection (n = 10), defined as relative binding compared to pooled human serum. Statistical analysis was performed using a Mann-Whitney test, to compare either IgG or IgM binding between healthy donors and patients.

(D) Spearman correlation between IgM binding to Newman Δspa/sbi and cumulative IgM binding to TarS- and TarM-WTA beads; patients (n = 10) are shown in blue and healthy donors (n = 11) in black. Dotted line represents the lower limit of quantification and symbols shown below the line represent extrapolated values. ns, non-significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. See also Figure S4.