Association between longitudinal WTA-specific antibody responses and ICU mortality in patients with S. aureus bacteremia
(A and B) Normalized binding of plasma-derived WTA-specific (A) IgG2 and (B) IgM according to the three WTA glycotypes from ICU patients with S. aureus bacteremia, stratified by ICU mortality. Boxplots extend from the 25th to 75th percentiles, and whiskers represent the total range. Symbols indicate individual patients with S. aureus infection (n = 36). Statistical analysis was performed using a Mann-Whitney test.
(C) Cumulative IgM binding for the three WTA glycotypes (TarS-, TarP-, and TarM-WTA) in longitudinal plasma samples (in days from positive blood culture) for ten patients with S. aureus bacteremia. Plasma samples at time point zero coincides with plasma samples shown in Figures 3A/B and 4A/B. The shading indicates patients that died in the ICU (p12, p19, p20, and p35). # refers to a different timing of sample acquisition (not −7 days). Dotted line represents the lower limit of quantification and symbols shown below the line represent extrapolated values. See also Figure S5. ns, non-significant, ∗p < 0.05, ∗∗p < 0.01.