REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor 647-conjugated goat F(ab’)2 -anti human kappa	Southern Biotech	Cat # 2062-31; RRID: AB_2795742
PE-conjugated goat anti-human IgG	Southern Biotech	Cat # 2040-09; RRID: AB_2795648
FITC-conjugated goat anti-human IgM	Southern Biotech	Cat # 2020-02; RRID: AB_2795600
Alexa Fluor 647-conjugated goat F(ab’)2 anti-human IgA	Southern Biotech	Cat # 2052-31; RRID: AB_2795711
PE-conjugated mouse anti-human IgG1 Fc	Southern Biotech	Cat # 9054-09; RRID: AB_2796628
Alexa Fluor 488-conjugated mouse anti-human IgG2 Fc	Southern Biotech	Cat # 9070-30; RRID: AB_2796640
Alexa Fluor 647-conjugated mouse anti-human IgG3 hinge	Southern Biotech	Cat # 9210-31; RRID: AB_2796703
FITC-conjugated rabbit F(ab’)2 anti-human C3c	Dako, Cruz et al.13	Cat #F0201; RRID: AB_2335709
FITC-conjugated rabbit F(ab’)2 anti-human C1q	Dako, Cruz et al.13	Cat #F025402
PE-conjugated goat F(ab’)2 anti-human IgM	Southern Biotech	Cat # 2022-09; RRID: AB_2795614
Alexa Fluor 647-conjugated goat anti-human IgG	Southern Biotech	Cat # 2040-31; RRID: AB_2795651
sheep anti-human IgG	ICN Biomedicals	Cat # 682311; RRID: AB_2335117
sheep anti-human IgM	ICN Biomedicals	Cat # 682331
ChromPure human IgG	Jackson Immunoresearch	Cat # 009-000-003; RRID: AB_2337043
ChromPure human IgM	Jackson Immunoresearch	Cat # 009-000-012; RRID: AB_2337048
horseradish peroxidase (HRP)-conjugated goat anti-human IgG	Southern Biotech	Cat # 2040-05; RRID: AB_2795644
horseradish peroxidase (HRP)-conjugated goat anti-human IgM	Southern Biotech	Cat # 2020-05; RRID: AB_2795603
Bacterial strains
Staphylococcus aureus N315 wildtype (WT)	NARSA strain collection	www.narsa.net
S. aureus N315 Δspa	Gerlach et al.22	N/A
S. aureus Newman WT	ATCC	Cat # 13420
S. aureus Newman ΔspaΔsbi	Sibbald et al.50	N/A
S. aureus Newman ΔspaΔsbi - mAmetrine	Cruz et al.12	N/A
S. aureus MW2 WT	CDC51	N/A
S. aureus NRS384 WT	NARSA strain collection	www.narsa.net
S. aureus NRS384 ΔtarM	Winstel et al.32	N/A
S. aureus 8325-4 WT	NARSA strain collection	www.narsa.net
S. aureus RN4220 ΔtarS	Winstel et al.52	N/A
S. aureus RN4220 ΔtarM	Winstel et al.52	N/A
S. aureus clinical isolates	This study	N/A
Biological samples
IgG- and IgM-depleted human serum	Zwarthoff et al.53	N/A
Baby rabbit serum	Pel Freez	Cat # 31061
Bovine serum albumin	Serva	Cat # 11930
Human serum albumin (Albuman)	Prothya Biosolutions	RVG: 103585
Chemicals, peptides, and recombinant proteins
UDP-GlcNAc	Merck	Cat #U4375
protein A from S. aureus	Cruz et al.13	N/A
CHIPS from S. aureus	De Haas er al.31	N/A
TMB substrate for ELISA	Sigma	Cat #T0440
RPMI Medium 1640(1x)	ThermoFisher Scientific	Cat # 52400-025
Polymorphprep	Alere Technologies	Cat # 1114683
Achromopeptidase	Sigma	Cat # A3547
Lysostaphin	Sigma	Cat #L9043
PEI max	Polysciences	Cat # 24765
POROS™ CaptureSelect™ IgM Affinity Matrix	ThermoFisher Scientific	Cat # 2812892005
Jacalin Agarose	ThermoFisher Scientific	Cat # 20395
Critical commercial assays
NucleoBond Xtra Midi kit	Macherey-Nagel	Cat # 740410
Experimental models: Organisms/strains
Mus Musculus BALB/c	Charles River	Strain # BALB/cAnNCrl
Oligonucleotides
Forward TarS: 5′- GTGAACATATGAGTAGTGCGTA-3′	Integrated DNA Technologies	N/A
Reverse TarS: 5′-CATAATGTCCTTCGCCAATCAT-3′	Integrated DNA Technologies	N/A
Forward TarM: 5′-GGGATACCCATATATTTCAAGG-3′	Integrated DNA Technologies	N/A
Reverse TarM: 5′- CAATTCGCTTCGTTGGTACCATTC-3′	Integrated DNA Technologies	N/A
Software and algorithms
GraphPad Prism 10	GraphPad Software	https://www.graphpad.com/scientificsoftware/prism/
FlowJo (v.10.8.1)	FlowJo	https://www.flowjo.com/
Other
Dynabeads™ M-280 Streptavidin	ThermoFisher Scientific	Cat # 11205D